Margaret Knyvett, (also spelt Knyvet), of Charlton by Malmesbury was born in Charlton by Malmesbury, Wiltshire, England and married Henry abt 1558.

Anne was seduced by Edward De Vere, (17th Earl of Oxford). The resulting, illegitimate child led to a led to a long-running feud between her uncle, Lord Thomas and De Vere. It resulted in the deaths of three followers of De Vere and Lord Thomas as well as injury to both men. The infant son was buried on May 9 1583.